sureprint g3 mouse ge 8 60k, v2 microarrays  (Agilent technologies)

Journal: Immunity

Article Title: Ablation of transcription factor IRF4 promotes transplant acceptance by driving allogenic CD4 + T cell dysfunction

doi: 10.1016/j.immuni.2017.11.003

Figure Lengend Snippet: (A) Flow cytometry analysis of cell surface molecules expressed on naïve WT CD4+ T cells (gray shades), or on activated WT (black lines) or Irf4−/− (red lines) CD4+ T cells one day after stimulation with B6 APCs (mitomycin C-treated, T-cell–depleted B6 splenocytes) and soluble anti-CD3 mAb. (B-E) WT and Irf4−/− CD4+ T cells were activated for 2 days. RNA was analyzed by microarray and quantitative real-time PCR, and Helios expression was analyzed by flow cytometry. (B) Heat map showing the normalized expression scores (relative to row mean) of selected genes from WT or Irf4−/− CD4+ T cells. Two RNA samples of each group were obtained from two independent culture experiments of pooled T cells from n = 3 mice per sample. (C) GO categories enrichment analysis of 438 upregulated genes in Irf4−/− CD4+ T cells in accordance with biological process. The horizontal axis shows −log10 of the P-value. (D) Relative changes of mRNA expression of the indicated genes in Irf4−/− CD4+ T cells compared to WT CD4+ T cells determined by quantitative real-time PCR. Data are mean ± SD. (E) Flow cytometry analysis of Helios expression in WT and Irf4−/− CD4+ T cells. Data are representative of three experiments with triplicate samples (A, D, E).

Article Snippet: Cyanine-3 labeled cRNA was prepared from 0.5 μg RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent Technologies) and hybridized to Agilent SurePrint G3 Mouse GE v2 8×60K Microarray (G4852B; 074809).

Techniques: Flow Cytometry, Microarray, Real-time Polymerase Chain Reaction, Expressing

Journal: Immunity

Article Title: Ablation of transcription factor IRF4 promotes transplant acceptance by driving allogenic CD4 + T cell dysfunction

doi: 10.1016/j.immuni.2017.11.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cyanine-3 labeled cRNA was prepared from 0.5 μg RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent Technologies) and hybridized to Agilent SurePrint G3 Mouse GE v2 8×60K Microarray (G4852B; 074809).

Techniques: Labeling, Plasmid Preparation, Purification, Recombinant, Staining, Cell Isolation, Microarray, shRNA, Sequencing, Software